does RSEM need STAR?
Hi, I'd like to estimate gene and isoform expression levels from RNA-Seq data with RSEM. Can it work alone (rsem-prepare-reference and rsem-calculate-expression) or does it need to be associated with the STAR aligner? in other words do I have first to produce bam files with STAR ans then perform a rsem-calculate-expression? Thanks!
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Francois Piumi •
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Francois Piumi •
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You'll need to point RSEM to either STAR or bowtie - it will then use that aligner before calculating expression.