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Any suggestions for cultivar identification using SSR (simple sequence repeat) markers

This is the data I have now: 30 SSR markers for 80 cultivars of cucumber. 10 of the 80 cultivars belong to one cultivar (let's say A).

My goal is that when a person hand me a new unknown cultivar I can tell the person whether it's A or not after I genotype the new cultivar using 30 SSR markers.

I'm think of considering it as a classification problem (A vs non-A) and use machine learning method to build a model using the SSR markers as features. But the problem is that A cultivars don't have enough number of samples.

Do you have any suggestions which statistical method(s) I can try to solve this problem? Thanks in advance.

ssr machinelearning marker

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