Problems setting up nt blast database

Question

[goatsrunfaster]

I am pulling my hair out trying to simply set up blast on my university server system. I downloaded all 36 of the packaged nt database files from the ncbi ftp site (nt.00-nt.35), and put them in the following folder: /network/rit/lab/andamlab/bin/ncbi-blast-2.10.1+/blast/db

I then added this path to my bash profile, but when I run the following:

blastn -db db -query GCF_006334995.1_ASM633499v1_genomic.fna -out blast_results.out

I get this:

BLAST Database error: No alias or index file found for nucleotide database [db] in search path [/network/rit/lab/andamlab/jrmatthews/UNH_Data_Streptomyces/streptomyces:/network/rit/lab/andamlab/bin/ncbi-blast-2.10.1+/blast/db:]

The db folder contains no subdirectories and everything is unpacked, I have also confirmed there is a .nal file there as well? What am I missing here?

Any help is greatly appreciated!

Answer 1

[goatsrunfaster]

I figured it out, I didnt realize the -db option had to be the .nal file name (which in this case was env_nt)