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Looking for raw, unprocessed RNA-seq datasets

Hi everyone,

I am looking for raw, unprocessed RNA-seq data to test a couple of things on.

I am trying to find one dataset that consists of total RNA, and another that has been enriched for mRNA via poly-A selection.

The data should have the following properties:

  • Completely unprocessed: so no trimming, no rRNA removal, etc.
  • Preferably from within the last 2-3 years.
  • Paired-end.
  • Sequenced on an Illumina instrument.
  • At least 20 million reads.
  • Must be bulk RNA (so, not tissue specific).
  • Preferably from a model organism (not too important).

Does anyone have any suggestions? I'm trawling through the SRA at the moment, but perhaps somebody here has some ideas as well.

rna-seq dataset raw reads data
• 1,265 views • link
written by Dunois • Dunois

1 answer

the new ENA browser has quite advanced query possibilities https://www.ebi.ac.uk/ena/browser/advanced-search , you might give that one a try?

• 0 views • link
written by lieven.sterck lieven.sterck

Oh wow, didn't know ENA had that. That's just amazing! Thank you!

• 0 views • link
written by Dunois • Dunois

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