Looking for raw, unprocessed RNA-seq datasets

Question

[Dunois]

Hi everyone,

I am looking for raw, unprocessed RNA-seq data to test a couple of things on.

I am trying to find one dataset that consists of total RNA, and another that has been enriched for mRNA via poly-A selection.

The data should have the following properties:

• Completely unprocessed: so no trimming, no rRNA removal, etc.
• Preferably from within the last 2-3 years.
• Paired-end.
• Sequenced on an Illumina instrument.
• At least 20 million reads.
• Must be bulk RNA (so, not tissue specific).
• Preferably from a model organism (not too important).

Does anyone have any suggestions? I'm trawling through the SRA at the moment, but perhaps somebody here has some ideas as well.

Answer 1

[lieven.sterck]

the new ENA browser has quite advanced query possibilities https://www.ebi.ac.uk/ena/browser/advanced-search , you might give that one a try?

[Dunois]

Oh wow, didn't know ENA had that. That's just amazing! Thank you!