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Number of normal samples necessary for creating the reference in case of WGS

Hi, I will be testing the CNVKit for sWGS (and ctDNA) and I would like to know if there is a minimum number of samples to create the reference pool for the same?

In addition if we don't use a normal panel and use a flat reference instead, how will it impact in this situation?

Thank you in advance.

cnvkit

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