finding transgene presence in the genome
In a review we were asked to show transgene presence in the genome of our organism (bird). The transgene is constructed of a human PGK gene plus a GFP sequence and has the length of ~600bp.
I was wondering what options we have to first find out if the transgene was integrated in the genome and second what tools can I use to try and locate the transgene insertion regions.
For the first point - would it be enough to map the genome against the human sequence to see if I can identify the hits against the hPGK?
Does anyone has experience with the TLA workflow? would this might be a ggood solution to identify the location of both ends of insertion?
thanks
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Assa Yeroslaviz
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