Kallisto out put be analyzed using Limma-Voom
Dear All,
Can the estimated counts of Kallisto out put be analyzed using Limma-Voom.
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ATpoint
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dr.fatemeh.askari •
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dr.fatemeh.askari •
1 answer
Sure but since kallisto produces transcript level abundance estimates and limma-voom was designed for gene level DE analysis you need to aggregate the transcript level estimates to the gene level, see https://bioconductor.org/packages/release/bioc/vignettes/tximport/inst/doc/tximport.html#kallisto
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