Hi there,

I have some RNA-seq data, both bulk and SC. I want to infer something from the bulk sequencing and then validate through scRNA-seq. I am studying the microenvironment of cancer, and so I would like to decipher possible extracellular signals given a set of up-regulated genes.

I have had a look at some upstream regulator apps from cytoscape (e.g PathLinker) but these do not seem to have the option to have possible receptor activators.

For example, given upregulated STAT5, JAK3/1, AKT and PI3K, I would like to be able to infer possible extracellular signals that trigger the given pathways, e.g IL-7/IL-7Ralpha.

Could you suggest any tools that could do this? Be it web based, R or Python.

Thanks.