Mapping >70%, very few counts in matrix
Hi,
I have mapped reads to an assembled reference, and generated a count matrix.
Is there are way I can check for a reason my count matrix has 6/91000 counted transcripts, though the mapping percentage is high?
Thanks,
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Check the 6 genes - what are they and why might they be accounting for so many of your reads? We can't help you beyond that without the code used for alignment and count generation.