Hi,

I am trying to create a heatmap of differentially expressed lncRNAs in normal, atypical, dcis, and invasive breast cancer patient samples. I have created a heatmap from the counts file but it creates a color map based of the counts of highest or lowest expressed RNAs not in that specific row but in general. So a specific lncRNA is expressed 100 times in cancer, but that lncRNA is only expressed 20 times in normal, it still shows blue as a color becausec some other lncRNA represented in a different row is expressed a 1000 times. I want to create a color map where the color coordinates to the expression of that specific RNA in that row across the different conditions. So a specific lncRNA is expressed 100 times in cancer, but that lncRNA is only expressed 20 times in normal, it should show red for cancer vs blue for normal. Basically, I want the scale or the heatmap be relative to each row across the four different conditions for one lncRNA. Any help is appreciated.