Trimmomatic on NEBNext Ultra II FS DNA
Hello together,
has anybody used Trimmomatic with the NEBNext Ultra II FS DNA Lib Prep Kit (NEBNext Ultra II FS DNA) together with Multiplex Oligos from NEB (e. g. Multiplex Oligos for Illumina) before? Do I need to adjust the reference adapter file of Trimmomatic? Since the adapters used by NEB are the same as of Illumina's TruSeq, I guess there is no adjustment needed. Am I right?
• 1,877 views
•
link
written
by
mario_de_manner •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Issue with Agilent 2100 BioAnalyzer HS DNA Assay Trace
written by Beatrice •
-
fastqc: Per base sequence content
written by khoojj •
-
Exact duplicated reads between independent replicates
written by Miguel •
-
Adapter sequences in RNA-seq
written by artgrigorov •
-
Trim NEBnext Adapters with trimmomatic - Same as TruSeq adapters?
written by alexgoro13 •
-
Truseq Stranded Total RNA and NEBNext Ultra II Directional RNA library prep kit
written by deniz •
-
Low percentage of Illumina reads retained after trimming with Trimmomatic
written by Bea •
-
has anyone use trimmomatic with NEBNext adaptors
written by eozcan •
-
Adapter trimming of small RNAseq data
written by MAPK
-
Screening Illumina reads for contamination
written by T_18 •
If what you say is true then I would assume you are good to go. However, it would be good to double-check post trimming that there aren't specific oligos remaining at either read end (e.g. fastqc).