Any other way to validate RNA-seq data if qPCR tests is not available?
I have some sequencing data which has been computationally analyzed for GO, and Differential expression analysis. Unfortunately,qPCR wasn't done for the samples. Is there any way/method we can confirm our findings are validated?
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Kevin Blighe
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I would try to replicate the findings in publicly-available datasets. You will likely find some data on Gene Expression Omnibus (GEO).
Kevin
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Relevant twitter thread:
https://twitter.com/andy_utoronto/status/1266144011259195394