I would like to filter only mapped reads with hight MAPQ.
I use bowtie2
What value should i give to MAPQ? i would like to make variant calling after filtering good reads from bam.
Hi, I aligned my paired-end sequencing data using Bowtie2 and processed the resulting BAM files with Picard MarkDuplicates. However, I noticed a discrepancy between the …
Hi everyone! I've encountered an interesting discrepancy when trying to filter the results of my paired end CHIP-SEQ. When using bowtie2 in Galaxy interface it …