AmpliSeq Thermo Fisher Sequencing · Biostar

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AmpliSeq Thermo Fisher Sequencing

Hi, I have an experiment that was run using AmpliSeq Thermo Fisher Sequencer. The output was Reads Per Million. I am not very much familiar with AmpliSeq technology. I am not sure if I need apply any sort of normalization technique here. Currently I did log2(RPM+1) transformation to make them log2 transformed. Is the methodology correct? Or am I missing anything here. The goal is to run the differential gene expression analysis and counts are not available and I don't have access to raw data. Any help is much appreciated.

Best, Prathyusha

rna-seq ampliseq normalization

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written 6.1 years ago by prathyushareddy87 •

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