Biostar Beta. Not for public use.
Pca From Vcf Files
12
Entering edit mode
6.5 years ago
Rubal7 • 760

Can anyone recommend a good software for doing Principal Component Analysis from data in VCF file format, or the most straightforward format to convert the VCF into for doing PCA. I hear that Plink is quite suitable for this. I also have some experience using eigenstrat for SNP data but have no experience using eigenstrat with whole genome VCF encoded data. Any tips or experience appreciated.

Many thanks,

Rubal

pca vcf genome • 19k views
ADD COMMENTlink
1
Entering edit mode
ADD REPLYlink
0
Entering edit mode

I agree, it would be nice to have a tool that does this. Meanwhile, You could create your own file with values of 0, 1, or 2 for homozygous ref, het, homoz alt. Then it'd be simple to use a standard PCA library to do the reduction.

ADD REPLYlink
16
Entering edit mode
19 months ago
United States

You can use VCFtools to make a PED and MAP file from VCF. This is PLINK format. Many PCA programs take PLINK input or offer conversion scripts.

I ended up using SNPRelate. After some silly errors here is how I got it to work:

setwd("/xxx/pca")
library("SNPRelate")
vcf.fn<-"~/xxx/tmp.vcf"
snpgdsVCF2GDS(vcf.fn, "ccm.gds",  method="biallelic.only")
genofile <- openfn.gds("ccm.gds")
ccm_pca<-snpgdsPCA(genofile)
plot(ccm_pca$eigenvect[,1],ccm_pca$eigenvect[,2] ,col=as.numeric(substr(ccm_pca$sample, 1,3) == 'CCM')+3, pch=2)
ADD COMMENTlink
0
Entering edit mode

1000 Genomes also has a tool for producing plink http://www.1000genomes.org/vcf-ped-converter

ADD REPLYlink
0
Entering edit mode

tried it, and after ccm_pca<-snpgdsPCA(genofile) got: Removing 3604 non-autosomal SNPs. Error in snpgdsPCA(genofile) : There is no SNP!. Any idea?

ADD REPLYlink
2
Entering edit mode

Experienced the same issue. It seem the problem is that by default, chromosome names are not in the form "chr1" etc., but just "1" etc. The solution is to use function snpgdsOption() to redefine your chromosome names to whatever form they are in your vcf file : snpgdsVCF2GDS(vcf, "ccm.gds", method="copy.num.of.ref", option=snpgdsOption(chr1=1, chr2=2, chr3=3, chr4=4, chr5=5, chr6=6, chr7=7, chr8=8, chr9=9, chr10=10, chr11=11, chr12=12, chr13=13, chr14=14, chr15=15, chr16=16, chr17=17, chr18=18, chr19=19, chr20=20, chr21=21, chr22=22, chrX=23, chrY=24, chrM=25))

Another solution is to add autosome.only=FALSE in snpgdsPCA() - it then takes all your chromosomes whatever their names are.

ADD REPLYlink
2
Entering edit mode

you meant autosome.only=FALSE ? since TRUE returns the same error ("Removing 362090 non-autosomal SNPs. - there is no SNP")

ADD REPLYlink
0
Entering edit mode

that works. thx!

ADD REPLYlink
0
Entering edit mode

Hi All,

Can you please explain about this line:

plot(ccm_pca$eigenvect[,1],ccm_pca$eigenvect[,2] ,col=as.numeric(substr(ccm_pca$sample, 1,3) == 'CCM')+3, pch=2)
ADD REPLYlink
0
Entering edit mode
ccm_pca$eigenvect[,1],ccm_pca$eigenvect[,2] implies that you are plotting between eigen vectors 1 and eigen vectors 2 ... PC1 and PC2...
ADD REPLYlink
0
Entering edit mode

Thank you - this was very helpful. After struggling with Eigenstrat, I managed to produce a nice graph with this R package. I am relatively new to R and this is my question: Is there a way of labelling the different individuals in order to be able to distinguish the outliers in the graph?

ADD REPLYlink
0
Entering edit mode

Have you find how to manage it ?

ADD REPLYlink
0
Entering edit mode

I was able to run the PCA without errors and I got a nice plot. But I want to specify my two populations. How can I create the population file? Or do I need to add this info in the GDS file? Thanks!

ADD REPLYlink
0
Entering edit mode

Hi, I found this thread really helpful . My data is grouped in 4 different populations and I managed to get them labelled as such by doing something similar to the following. Following on from Zev's code above....

>genofile <- snpgdsOpen("ccm.gds")
>sample.id <- read.gdsn(index.gdsn(genofile, "sample.id"))
>sample.id
[1] "Sample1"   
[2] "Sanple2"  
[3] "Sample3"  
[4] "Sample4" 
[5] "Sample5"

In a text file, list the group name of each sample in sample.id, one group per line with each line corresponding to the group of the corresponding sample. For example if Sample 1 and 2 in sample.id were in 'Group 1' and Sample 3 - 5 were in 'Group 2', you'd have:

Group1
Group1
Group2
Group2
Group2

Save the file as 'pops.txt' and then:

>pop_code <- scan("pops.txt", what=character())
>cbind( sample.id, pop_code)

>ccm_pca<-snpgdsPCA(genofile, autosome.only=FALSE, num.thread=4)

>tab <- data.framesample.id = ccm_pca$sample.id,
              pop = factor(pop_code)[match(ccm_pca$sample.id, sample.id)],
              EV1 = ccm_pca$eigenvect[,1],    # the first eigenvector
              EV2 = ccm_pca$eigenvect[,2],    # the second eigenvector
              stringsAsFactors = FALSE)

>plot(tab$EV2, tab$EV1, col=as.integer(tab$pop), xlab="eigenvector 2", ylab="eigenvector 1")
legend("bottomright", legend=levels(tab$pop), pch="o", col=1:nlevels(tab$pop))

And that should do it!

ADD REPLYlink
0
Entering edit mode

Just tried it but got some error...

tab <- data.framesample.id = ccm_pca$sample.id Error in (tab <- data.framesample.id) = ccm_pca$sample.id : object 'tab' not found

What is data.framesample.id exactly?

ADD REPLYlink
11
Entering edit mode
16 months ago
sa9 • 800
USA, Cambridge

SNPRelate is an R package that is able to read from VCF files directly and perform PCA and IBD/IBS. According to the documentation, it runs 10-45x faster than EIGENSTRAT (v3.0) and PLINK (v1.07) respectively.

Update (Oct 2014): The package seems to be moved to GitHub (link)

ADD COMMENTlink
0
Entering edit mode

thanks, that's a good find!

ADD REPLYlink
0
Entering edit mode

Just tried it. It hates my Unified genotyper VCF??

ADD REPLYlink
1
Entering edit mode

What is the error message? Can you post part of the VCF?

ADD REPLYlink
0
Entering edit mode

There was no problem with the function, it was user error :-).

ADD REPLYlink
0
Entering edit mode

Can you elaborate? I get "file has different number of columns" with a UnifiedGenotyper VCF. Is there a fix, or this type of VCF not suitable?

ADD REPLYlink
0
Entering edit mode

Neilfws I used a multi-individual Unified Genotyper VCF as an input. I can't quite remember what the error was, but I can remember how I got it to work. See revised post above.

ADD REPLYlink
0
Entering edit mode

Thanks for that. I had no joy with the VCF; I converted to PLINK bed format using vcftools then used snpgdsBED2GDS() in SNPRelate. That converted to GDS no problem.

ADD REPLYlink
0
Entering edit mode

This sounds great

ADD REPLYlink
0
Entering edit mode

which version if R can be ran into? I got this error:

library("SNPRelate") Error in library("SNPRelate") : there is no package called ‘SNPRelate’ install.packages("SNPRelate") Installing package into ‘/Users/ib7/Library/R/3.4/library’ (as ‘lib’ is unspecified) Warning in install.packages : package ‘SNPRelate’ is not available (for R version 3.4.2)

any advice? thanks

ibseq

ADD REPLYlink
0
Entering edit mode

SNPRelate has been removed from CRAN. You need to install it from Bioconductor now.

ADD REPLYlink
0
Entering edit mode

How can we add label in PCA plot generated by snprelate to see samples?

ADD REPLYlink
4
Entering edit mode
17 months ago
mkulecka • 300
European Union

While this thread in very old, I think it would be useful to add that PLINK now directly supports vcfs link to new (1.9) version of PLINK. .

ADD COMMENTlink
0
Entering edit mode

It makes things easier than ever. plink --pca --allow-extra-chr --vcf samples.vcf

ADD REPLYlink
0
Entering edit mode

How would you plot the resulting output?

ADD REPLYlink
0
Entering edit mode

With R it's pretty easy:


library(ggplot2)
library("ggrepel")

df<-read.delim("plink.eigenvec") #read in eigenvectors

eigens<-read.delim("plink.eigenval",header=F) #read in eignevalues

sum_eig<-sum(eigens$V1)

sum_eigs<-lapply(eigens$V1,function(x){ rt<-(x/sum_eig)*100 rt<-round(rt) return(rt) })

ggplot(df, aes(PC1, PC2, color=condition)) + geom_point(size=3) + xlab(paste0("PC1: ",sum_eigs[[1]],"% variance")) + ylab(paste0("PC2: ",sum_eigs[[2]],"% variance"))+geom_text_repel(aes(label=SampleID), size=3) #The part with geom_text_repel adds easy-readable labels.

I have slightly modified my eigenvectors file I have added SampleID and condition columns.

ADD REPLYlink

Login before adding your answer.

Similar Posts
Loading Similar Posts
Powered by the version 2.3.1