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uORF annotation in Ensembl/GENCODE

Do protein coding genes in Ensembl/Gencode GTF files always only have one CDS? For example if a protein has a uORF that has been demonstrated to be translated, will it be annotated as a second CDS in the transcript, or can I always assume that the 5' most start codon in a GTF record is the start of the main CDS?

annotation ensembl gencode uorf

1 answer

As far I know, uORF should be annotated as a new CDS for the protein.

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