great response. thank you
• 0 views
•
link
written
by
askari.arezou94 •
diffrent between CNV and pcr duplicate
Hi guys, How can distinguish the difference between Copy Number Variation and PCR duplicate in sequencing out put???
• 1,729 views
•
link
written
by
askari.arezou94 •
1 answer
PCR duplicates are a result of a DNA fragment that was used to prepare the library has been sequenced multiple times.
CNVs are changes in the actual number of copies of a section of DNA in the cells being sequenced.
These are fundamentally different concepts. CNVs are identifed based on the aggregate coverage (and/or variant allele fraction) of the genome. PCR duplicate just affect one DNA fragment in the library. CNVs reflect underlying biology. PCR duplicates are technical artefacts of sequencing library preparations that use PCR amplification.
Log in to answer this question.
More posts like this
-
Inquiry about depth of coverage and copy number variation
written by Chen •
-
validation copy number variations in whole genomes
written by askari.arezou94 •
-
PCR duplicates and sequence duplicates
written by Sujata •
-
DNA methylation 450k data - minfi - preprocessFunnorm - CNV cases vs non-CNV controls
written by Thomas •
-
Input ChIP-seq coverage changes at centromere/telomeres
written by Carlo Yague
-
PCR duplicata and de novo assembly ??
written by Picasa
-
What absolute copy number would you call an Amplification?
written by senowinski •
-
Copy number variation detection tools within exome sequencing
written by mangfu100
-
Copy number variation subtypes
written by neenu.abraham8 •
-
Copy Number Analysis V/S Differential Gene Expression
written by Abhi
I think PCR duplicates are handled early on in pipelines, using tools such as Picard's MarkDuplicates. My knowledge on this matter is quite outdated though, so you should wait for others' inputs and/or do some searching on your own.
ok, tnx for your response.
great response thank you.