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validation copy number variations in whole genomes

Hello every one I have a question about the accuracy of copy number variations found in the whole genome how can I validation copy number variations found in the laboratory? which method do you offer for me?

best regards

genome sequence assembly

You can easily use FISH or MLPA

Thank you for your response can I use from cloning for confirming validation?

I believe it's possible but never used this approach! Also the qPCR, never used it to validate CNVs.

ook, thank you. actually, I thought coloring and qPCR are the one way to validate CNVs. do you know , why never used from qPCR and Cloning to confirm copy number variations?

We have a pathology lab integrated, so every CNV is confirmed by them using FISH (for somatic). For germline CNVs we use MLPA in the lab.

Excuseme, Can i use from "gfp Crisper" for validation copy number variations?

1 answer

Can I use from qPCR to confirming copy number variations?

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