demultiplex PacBio fastq file
Hi everyone,
I have just got back my PacBio sequencing of fungal ITS2 amplicons. I received one fastq file and I need to demultiplex the data in order to perform the filtering the clustering. I have never performed demultiplexing so I would like to ask which pipeline could I use to demultiplex this dataset.
Thanks a lot for any help!
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Are barcodes in the header? Please add details. How many samples are in the file?
For PacBio data you can try their demultiplexing software LIMA.
Someone posted this a couple of days back: A: How to demultiplex PacBio from CCS.h5 or fastq You will need to see if this is correct.
You can also find a google group "SMRT_isoseq" to ask there specific questions.