I am trying to retrieve the 3'UTRs for the list of gene ids from this dataset:
http://cbio.mskcc.org/saturation/NBT-RA20689B/NBT-RA20689B_SuppTable1.xls).
I have managed to get the sequences for about 1300 (out of ~20000) with Biomart using DBASS5 Gene Name as filter.
I have also used the Table Browser from UCSC..Some of the id's (~3000, not sure which) are not compatible with the refseq gene ids from their repository. The ids returned are in the following format:
hg19refGeneNM_032291 range=chr1:67208779-67210768 5'pad=0 3'pad=0 strand=+ repeatMasking=none
, and since I do not know which ones are not valid I cannot map them back to my gene set
How can I get the complete list of 3UTRs for this gene list?
biomart
ucsc
ensembl
A little confused as to how you retrieved UTRs for 1300 genes using only one gene name? Perhaps describe more exactly what you did in BioMart?
I have uploaded a file containing the gene names..That is the filter type ..for the type of genes, in the drop down list of the Filters/ID_list_limit. Unfortunately, the data contains some gene ids which are not recognized by that filter. I have uploaded them in the Genome Browser and apparently around 3000 of them are not recognized as ref seq gene ids
Are you interested in pulling out the sequences of the actual 3'UTR or would it suffice to retrieve a specific length of sequence after every STOP in the coding region? If it's the latter, I can suggest a way to do it in galaxy.
I need the sequences for the actual UTR for motif finding .
i am interested to retrieve 3'UTR region from all the reported genes og buffalo. how can i do this?