Is PolyA RNA-seq equal to 3'Tagged RNA-seq?
Hi
Is PolyA RNA-seq equal to 3'Tagged RNA-seq?
Are those two the same thing?
James
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ATpoint
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u3005992 •
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u3005992 •
1 answer
No, polyA-based RNA-seq only means that transcripts were extracted that are polyadenylated. These can then be used for either full-length or 3'-tagged RNA-seq. 3'-tagged only means that the 3'-part of the transcript was enriched furing library prep in contrast to the entire fragmentated transcript/cDNA.
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