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What is the minimum sample size to get reliable results in haplotype and nucleotide estimations?

Different software have different methods for estimating diversity and each method will have assumptions about the quantity of data required to produce a reliable result. I am estimating the haplotype and nucleotide diversity by DnaSP6 program of Sanger sequences. I would like what is the minimum sample size requires to get reliable results.

Thanks in advance.

sequence

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