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Metagenome (Microbiome) composition and functional profiling

Hi there!!! I have several number of metagenomic 16S rRNA gene sequence. Firstly, I want to know the composition (i.e. all the species and strains out there in the metagenome) of the metagenome. Secondly, I will do the functional profiling.

  1. Can i use MOTHUR software for the first purpose?
  2. What should I do for the second one?

THANK YOU...

mothur 16s rrna metagenome microbiome
• 1,670 views • link
updated by Asaf Asaf • written by dpc dpc

1 answer

  1. Yes, you can use Mothur to do that. You can also use DADA2 which has some advantages over Mothur.
  2. You can use PiCrust. If it's mouse microbiome make sure you read this new publication: https://www.cell.com/cell-reports/fulltext/S2211-1247(20)30197-2
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written by Asaf Asaf

Thanks Sir for your response. I have installed and trial run MOTHUR. I am a newbie in bioinformatics and a little confused. So it would be a great help if you please in short tell me the steps to run MOTHUR for profiling the species composition and their abundances.

Regards

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written by dpc dpc

Thanks Sir. I wanted a protocol. And I have got one.

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written by dpc dpc

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