Hi, I'm a beginner in this field. I wish to download some cancer-related sequences to practice making Variant Calling Pipeline. Where can I find such sequences? Can you download such sequences from the SRA database??
1 answer
If you need 'raw' data FASTQ files and do not have access to such data at the TCGA or ICGC, then it would be easier to search for samples via:
Typically, searching at SRA first can be useful, but then downloading the data for these from ENA (by searching for the SRA BioProject ID at ENA). I say this because ENA makes the data available as FASTQ, whereas, with SRA, the data is encoded in a very annoying way. In relation to these, please see this tutorial: Fast download of FASTQ files from the European Nucleotide Archive (ENA)
Please also see these other answers:
Kevin
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