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ERROR Trinity: Error, no Inchworm output is detected

Hello,

I have this error when I try to run the assembly:

Error, no Inchworm output is detected at: /nfs/student/giovy92/RNA-seq/practice/trinity/trinity_out_dir/inchworm.K25.L25.DS.fa at //nfs/student/giovy92/RNA-seq/trinityrnaseq_r20140717/Trinity line 1279.

What does it mean?

The command that I have is

Trinity --seqType fq --JM 100G --CPU 8 --single ./combined.fastq.gz

Thank you!!!

rna-seq assembly

Which version of Trinity? Are there other error messages? Did you try the suggestions found at the many threads you find when searching for Error, no Inchworm output is detected?

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