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ONT fast5 basecalling

Greetings!

I have a lot of ONT sequencing data in .fast5 format and I need to perform a basecalling via guppy_basecaller. The problem is that computation will take about 1.5-2 months, because I don't have a decent GPU and have to perform the basecalling using CPU. The question is can I just split the data (from one sequencing run) and perform the computation on multiple PC's?

oxford nanopore fast5 sequencing basecalling
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updated by WouterDeCoster WouterDeCoster • written by a.lapin • a.lapin

1 answer

Yes, that's perfectly fine. If you can get access to a large cluster or server then that would also make things faster.

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written by WouterDeCoster WouterDeCoster

Thank you. Everything really worked well, the calculations took less than a week. But I have another question, where can I read something about quality scoring in ONT reads? I can not find any information about encoding.

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written by a.lapin • a.lapin

Hi @WouterDeCoster, do you have a guide for basecalling using Guppy ? (I don't have access to the community page)

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written by Picasa Picasa

What's unclear after reading the command line help?

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written by WouterDeCoster WouterDeCoster

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