Illumina RT primers in Ion RNA-seq?
Hi,
Once I perform fastQC in my RNA-seq data sequenced by Ion Total RNA-seq, some Illumina RT primers and ABI solid3 adapters appear as overrepresented sequences. Should I remove them from the study? Will they have an influence in the next steps of analysis (mapping/alignment...)?
Thanks,
JJ
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joaquinj12 •
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That seems quite odd, at what level were they present?
There are 772 counts and 0.23034255996515024 % for Illumina RNA RT Primer (100% over 8bp). For ABI Solid3 Adapter B (100% over 10bp), 417 counts and 0.12442078692418089 %.
That's low enough to ignore.