Hi Joe, You were right, Primer-Blast is working again! Many thanks for the advice anyway, I will contact NCBI help desk next time.
Dear all,
I am picking up primers through NCBI Primer-BLAST and it keeps returning the following error:
Exception error: No alias or index file found for nucleotide database [repeat/repeat_9989] in search path [/export/home/service/grid_nodes/primertool:/netmnt/blast/db/disk.blast/blast2150:/net/frosty/vol/blast/db/blast:] .
I am not sure it has anything to do with my parameters because I have tried the same genes and options previously and it has worked. I' ve looked through the forum and similar post only talk of this problem in BLAST+, but not on the web. It might be a temporary problem, but any idea of what might be going on? Thank you very much for your help.
1 answer
My guess would be that it may be a temporary problem, but this is likely something you'd need to speak to NCBI's helpdesk about, rather than the forum.
Log in to answer this question.
Sorry, the screenshot link is this one https://ibb.co/yQBYQ7K