Hi,

I have 25 vcf with only GT values, and I merged them with "vcf-merge ${all_samples}"

after merge I have some situations like the following where not all samples have a GT... in general is normal ...

CHROM   POS   ID   REF   ALT   QUAL   FILTER   INFO   FORMAT   wes001   wes002   wes003   ...   wes025

chr1    12807   .   C   T   39.94   .   AC=3;AN=4;SF=19,24  GT  .   .   .   .   .   .   .   .   .   .   .   .   .   .   ..  .   .   .   1/1 .   .   .   .   0/1

1) when I run bcftools roh with this code everything seems ok

bcftools roh -G30 merged.vcf.gz -o roh_merged.vcf.gz.txt"

With this code the number of roh in each sample are less here than when I run bcftools roh in eah sample separately, why? because I use "--AF-dflt 0.4"?

code for roh calling in each sample sepraterlly

bcftools roh -G30 --AF-dflt 0.4 "single_sample.vcf.gz

2) when I use this code, to calculate and use the AF on fly ...

 bcftools roh -G30 -e sample.list merged.vcf.gz -o roh_merged.vcf.gz.txt"

the command run without any result and after one hour it freeze!

The only log that I have are these:

Number of target samples: 25
Number of --estimate-AF samples: 25
Number of sites in the buffer/overlap: unlimited

sample.list are the sample names reported in the vcf (wes001, wes002) and here like a column, one name in each line...

 -e, --estimate-AF [TAG],<file>
estimate AF from FORMAT/TAG (GT or PL) of all samples ("-") or samples listed in <file>. If TAG is not given, the frequency is estimated from GT by default

Any help please! Best