Hi biostars. I'm trying to estimate the insert size of a pair-end illumina library. I have limited information about library preparation and sequencing protocol (2x125). …

Hi, I have difficulty in calculation for average insert size and standard deviation of RNA-seq NGS data to submit GEO database. I generated two fastq …

I want to use a paired end library for scaffolding my contigs. I used the following command to estimate insert size using bbmap: bbmap.sh ref=reference.fasta …

Hi all, I have obtained two illumina MiSeq 2x75 paired-end read files, one forward and one reverse. oenopla-reads1.fastq & oenopla-reads2.fastq Then I performed genome assembly …

-- Hello: in Galaxy anybody has had an experience like this? We're trying to map RNA-seq reads to a certified transcriptome from Vectorbase (bugs) but …

I am conducting a miso analysis for a single gene on a large number of Paired End RNAseq bams that is spread out on several …

I am trying to use VelvetOptimiser to do denovo assembly of a plant. I have 2 libraries; 100PE with insert size ~ 400bp and 150PE …

<p><img src='http://blog.malde.org/images/bam_plot.png' alt='enter image description here' /></p> <p>I want to visualise insert sizes in my mate-pair library and calculate statistics like mean, standard deviation..</p> <p>I …

<p>Hello,</p> <p>I have Illumina paired-end/mate-pair reads and I would like to find out the min and max insert size is. For Aligner like SOAP2 is …

<p>hello all,</p> <p>I have earlier worked with EST sequences, tools for assembly, gene finding ,etc. I am now interested in filling gaps about my knowledge …