You need to give us more information. A p-value is associated to a certain hypothesis/test. What are you testing here?

What are you interested in knowing using the read counts? If you have multiple samples, you could compute a differential expression analysis where your miRNAs would have a p-value based on the method you used (limma for example.)

i want to perform deferentially expression of genes and want to derive p value.i have file which has miRNA id and read counts. how can i derived p value from miRNA id and read counts ? please explain

let me clarify...working on lung cancer project and need to find out which genes are deferentially expressed and which are not. for that i a have downloaded miRNA data set from GEO id is GSE110907. And the file i got has to variable gene_id and read counts. from that i have to derive p value from that data/file.

example:

input file :

id              read_count
hsa-miR-100-3p  568
hsa-miR-100-5p  176883
hsa-miR-101-3p  122968
hsa-miR-101-5p  923

output file:

id              read_count          p value
hsa-miR-100-3p  568                 ?
hsa-miR-100-5p  176883              ?
hsa-miR-101-3p  122968              ?
hsa-miR-101-5p  923                 ?

husainbioinfos : Please edit your original post to add more/new information.

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