What is the difference between microRNAs with star and without star?
For example what's the difference between these 4 microRNAs? hsa-miR-101* hsa-miR-101 hsa-miR-101-3p hsa-miR-101-5p Which pairs (if) are the same? Which pairs are the same in sequence?
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miR-101 is the active miRNA, usually the 5p side. The star is the miRNA resulting from the other side of the hairpin, usually assumed to be non-active. This perspective has changed and now miRs are marked with 5p and 3p so usually hsa-miR-101 is hsa-miR-101-5p and hsa-miR-101* is hsa-miR-101-3p but you should check each case because sometimes the non-starred is the 3p.
Thank you very much for your answer, which is great for me. I've got some differentially expressed miRNAs, how do i know if his active miRNA is 3p or 5p? When i want to predict his target gene, should i use 3p or 5p?
For example, I would like to know whether the active form of hsa-miR-3613 is 5 or 3p? I went to miRbase view, found both forms have, I do not know which one is the active form? Can you teach me to distinguish? thank you very much
In this specific case they might both be active since they both have comparable amount of sequencing reads. There isn't a lot of other data regarding this specific miRNA. In the case of hsa-mir-101-1 it's much easier to see that the 3p has a lot more reads than the 5p and probably is the active part.
So I predict that the miR-3613 target gene should be the sum of the predicted 3p and 5p respectively? Because miRNA-name is only miRNA-3613 in TCGA. And no miRNA-3613-3p or miRNA-3613-5p
thank you all the same, you have helped me a lot
I wonder have you figured that out? I have same problem that TCGA does not provide 3p or 5p information. That makes me very hard to find gene target. Because of that I have been also looking through isoform information. However, now I am having difficulties about the normalization all 3p information or all 5p information. Please let me know if you figured that out. Thank you
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