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Conservation of Human and Mouse DNA sequences

Hello,

i have a list of DNA regions from mouse ESCs and I would like to know if these sequences are conserved also in Human. How can I do this?

comparative genomic conserved dna regions
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updated by JC JC • written by fusion.slope fusion.slope

1 answer

you can use UCSC GB LiftOver tool

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written by JC JC

do you think it is enough?

That you will need to decide.

If the right regions are present in the dataset you could also look at precomputed information available here from JAX.

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written by GenoMax GenoMax

What i am doing i am lifting the coordinates of mouse to human as also the coordinates of human to mouse. Then on the lifted coordinates i calculate the conservation score using phasCons in UCSC table browser. I simulate also a set of sequences with similar GC content, size and nucleotide composition of the lifted sequences and I compute again the conservation score. Then i compare the conservation scores of these files..

Does this make sense?

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written by fusion.slope fusion.slope

Not sure why you are doing this so can't say.

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written by GenoMax GenoMax

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