Hello. I am trying to cluster a huge fasta file containing using CD-HIT-EST with a threshold of 80%. According to the user's guide (http://www.bioinformatics.org/cd-hit/cd-hit-user-guide.pdf), I …

I would like to identify plastid derived sequences in the mitogenome. [The complete mitochondrial genome of the early flowering plant Nymphaea colorata is highly repetitive …

Hi everyone, I am performing tblastn with a set of >1000 proteins as queries against a genome. I am trying to keep every regions of …

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Hi everyone, I am trying to isolate a set of sequence ranging from 6 to 15 nucleotide in size from a single DNA sequence about …

Right now I'm trying a general blast for everything and then searching for the matches by taxid or the seqid's. I'm tried other approaches using …

Hi Everyone. I am trying to blast many short peptide sequences against a protein database. I am looking for nearly exact matches and selected word_size …

Hi guys, I’m trying to set up a COG database using the 2014 updated data (from ftp://ftp.ncbi.nih.gov/pub/COG/COG2014/). As I understand how it works, one needs …

Hi guys! Imagine, we are searching over distributed blast databases, slices of the one big non-redundant database. We run separate blast task over each slice …

I am having trouble getting blast to give me "correct" results. I am trying to retrieve as many hits with e-value better than 1. I …