Hi all As I am very new to the subject bioinformatics, I have few question which are listed below 1) How can i do blast for larger sequences, as in https://blast.ncbi.nlm.nih.gov/Blast.cgi it is not getting supported and i am unable to retrieved my blast file. 2) I want do the gene annotation of specific targeted region from my plant of interest (cotton), which software I should use. 3) Lastly I want to validate few gff3 files, which tool is suitable. Thank you in advance