This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Finding SNP between two genes sequenced by sanger sequencing

I have DNA sequences of a gene from two closely related Swallow species. I need to find the SNP between the two species. How can I do this without using a reference? The sequencing was done using Sanger sequencing Thank you in advance

snp

1 answer

I would use good old global alignment. needle from EMBOSS

Log in to answer this question.