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Differential Gene expression analysis on GEO data set

Hello everyone I am a Ph.D. student of Immunology have little knowledge of Bioinformatics, I have download raw data set from GEO and now want to do differential gene expression analysis in R, kindly guide me that what should I do step by step with this data to analyze it. here is the data GSE12124.

rna-seq r

1 answer

You may want to start with limma (R) and it's comprehensive manual.

If you have just been asked to process data with no guidance, then I feel for you; however, any good supervisor (if you have one) would at least give their student / staff member some low level guidance.

Kevin

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