How computationnaly verify a right ordering and orientation of a part of genome sequence with short reads illumina data
Hello everyone!
The whole genome alignment of a reference genome A (constructed with short reads + genetic linkage map) with a new genome B (short reads + pacbio long reads + Hi-C) revealed some inversion patterns in some chromosomes suggesting ordering and/or orientation problems. How can I elucidate the right ordering and orientation of the inverted region using short reads data? I want to know between A and B, which genome has the right orientation. Sincerely Yours.
• 837 views
•
link
written
by
angez9914 •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Plotting marker location co-linearity between multiple physical and genetic maps
written by William
-
Huge jump in plasmid coverage - any idea why?
written by predeus
-
Generate artificial reads
written by finswimmer
-
How to extend BAC sequences with Pacbio reads in order to rebuild a genomic region?
written by Thomas B. •
-
Interpretations of inversions in the output of MultiBreak-SV or GASV
written by loonreg •
-
[PacBio assembly] Remaining indels after polishing
written by Rox
-
How Reliable Are Sequencing Techniques Of Human Genomes With Oddities Like Missing Chromosome Arms?
written by jdimatteo •
-
How to validate translocation detected in RNA-seq data?
written by alisaber814 •
-
Coments On This Feature: Alignment Coordinate Projection From 1.Bam To 2.Bam To Create 3.Bam?
written by 2184687-1231-83-
-
Change Coordinates -A 1.Bam -B 2.Bam -O 3.Bam?
written by 2184687-1231-83-
