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help for WGCNA

i was followed the tutorial of WGCNA to run my data and try to learn about WGCNA,but there is some error i can not fix. i really need help, please!

> sampleTree2 = hclust(dist(datExpr), method = "average")
Error in hclust(dist(datExpr), method = "average") : 
  N must be at least 2.
rna-seq

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