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Error in WGCNA?

Hi Friends,

I am performing WGCNA in R. I am getting following error messages. Can anyone help me out to solve this error? Thanks 

> dissTOM = 1-TOM
Error: vector memory exhausted (limit reached?)
> dissTOM = 1-TOM
Error: vector memory exhausted (limit reached?)
> dissTOM = 1-TOM
Error: vector memory exhausted (limit reached?)
> dissTOM = 1-TOM
Error: vector memory exhausted (limit reached?)
> geneTree = hclust(as.dist(TOM), method = "average");
Error: vector memory exhausted (limit reached?)
> geneTree = hclust(as.dist(TOM), method = "average");
Error: vector memory exhausted (limit reached?)
> geneTree = hclust(as.dist(TOM), method = "average");
Error: vector memory exhausted (limit reached?)
> save.image("~/Desktop/Ambika_WGCNA/WGCNA_step2.RData")
Error: Unable to establish connection with R session

> geneTree = hclust(as.dist(TOM), method = "average");
Error: vector memory exhausted (limit reached?)
r rna-seq rna-seq
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updated by Kevin Blighe Kevin Blighe • written by harshraje19 • harshraje19

1 answer

You need a greater allocation of RAM / 'memory'. If you cannot allocate more RAM, try to reduce the input number of variables by removing low expressed genes and those with low variance.

Edit: your very first command could be due to another issue. Try to simply restart your computer in order to clear your cache. When you start a new analysis in R, always start a new R session.

Kevin

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written by Kevin Blighe Kevin Blighe

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