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How to distinguish reads with unique SNPs?

I'm now working on identifying mutation sites of C. elegans. And I'm using tools adapted from Galaxy. I'm wondering after crossing N2 background worms with Hawaiian worms, how could I distinguish certain read is from N2 worm or Hawaiian worm with SNP sites? I want some tools or methods described. Thanks a lot!

wgs genetics

Were these samples indexed? If so you should be able to identify which sample the read originated from by looking at its fastq header.

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