Mega X help - phylogenetic tree
Hi,
I'm VERY new to bioinformatics, and for my uni course I'm doing a project analysing the geographic spread of chytrid fungus. I have used NCBI to identify Batrachochytrium dendrobatidis (chytrid fungus) ITS1 & ITS2 partial sequences, I have saved these in a FASTA file and uploaded them to Mega x to produce a phylogenetic tree. However my out-groups are only out when I use UPGMA, the bootstrap values are mostly very bad and the tree doesn't look right at all, so I think I must be doing something wrong or maybe my sequences are bad. Just looking for some help and advice.
Thank you!!!
• 2,324 views
•
link
written
by
SILE1_17 •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
How to determine number of SNPs called?
written by SushiRoll •
-
emboss phylipnew fneighbor error reading large distance matrix into tree
written by vardee123 •
-
How to get gene sequences from non-model species for phylogenetic tree construction?
written by ges29 •
-
Bootstrap value with 0 as the lowest
written by sushi •
-
Correlating tree comparison results with genome properties
written by suchy •
-
How to incorporate outgroup organism for whole genome SNP based phylogeny?
written by Kumar
-
Exporting sequences from Excel
written by avocado_toast •
-
Phylogenetic tree construction with RAxML and low bootstrap values
written by vasilislenis
-
Making a phylogenetic tree for Galliformes
written by pseizure •
-
Treeio And Bootstrap Values
written by Lee Katz
Is it possible to provide a picture of the tree? Did you look at the genetic differentiation of the sequences between samples? Would you expect good bootstrap values or a good resolution of the tree based on the genetic differentiation of the samples at all?