Hello everyone, I am newbie using MAKER pipeline for genome annotation. I followed [this tutorial][1] to do the annotation for my assembled sequence. I generated …

Hi all, It's my first time trying to annotate an assembly, and I have been struggling with one aspect of Maker. When I run maker, …

I have recently used MAKER2 for genome annotation. The run appears to have been successful, but when I came to use `fasta_merge -d` there was …

Hello, I am using the software package called maker designed for genome annotation. Firstly, however, I need to convert a preexisting annotation set into a …

Hello everyone, I just finished my structural annotation on my genome using Maker and I wanted to start the functionnal annotation (= adding the biological …

I am trying to annotate my genome assembly using Maker. I have thousands of contigs, i discovered that few contigs failed during annotation. Since I …

Hi everyone, I recently have performed a **new genome annotation** with EVM, thanks to *ab initio* predictions, RNA-Seq data and homology protein alignments. As there …

Hi All, I tried using MAKER but the output files generated is empty, below is my command /data1/ngs/programs/maker/src/bin/maker2zff -n ../genome.all.gff -n option is used for …

Hi all, I am running Bowtie2 to get mapped reads and unmapped reads (--un-conc) from a paired-end experiment. My command line is like this: bowtie2 …

Hi all, I am using htseq-count for getting the read count of my genes from RNASeq data.While I have got the read counts but I …