I am fairly new to this field，and right now I am working with variant allele frequency for cancer genome,data is from cbioportal. I have read several articles and they claim mutant allele frequency can be used to estimate the intra tumor heterogeneity by looking at the number of cluster of those variant allele frequency, this is the part I am not understanding since those variant allele frequency is just measure of the variant allele divided by total allele, and it doesn't specify which gene is mutated. So could any one explain the connection to the how many clones the tumor has ? Thank you! Any help is appreciated!