Depth of a sequence reads
Hi, I am new to bioinformatics. I am reading a lot of papers to understand genome assembly. I have a question. Does high coverage of a read guarantee the nucleotide at a particular location? Thanks in advance!
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sindhujamohan92 •
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Read depth is a contributing factor, yes. Base quality also matters. You want to see, ideally speaking, quite a few reads aligning really well to a base position, where the aligning bases have been called at a good quality as well.