Only one enzyme for 4c-seq analysis, How to analyze ?
I have 4c-seq data from one enzyme experiment. But the pipelines I found use 2 enzymes for the analysis. Should I ignore 2nd enzyme or write the same enzyme in the configuration file?
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If you used specialized software you cannot simply ignore or tweak parameters while expecting adequate results. These tools/workflows assume standard library protocols with two enzymes. Is this some special 4C-seq protocol? Why only one enzyme?
I'm using 4cseqpipe. The data gave to me is only one enzyme.