I have WGBS pair-end fastq files. I am using Biscuit aligner. Is alignment stochastic? Can we get (slightly) different alignment from different runs on the same fastq files?
Changed your title to make it more specific, and added biscuit tag. Please think about this in the future, as such experts can more easily find your question.
Hi all, I am new to the field of paired-end reads analysis, and would appreciate your feedback regarding several issues in analysis of paired-end reads …
I am using [BISCUIT][1] for WGBS data. After the full process BISCUIT provides bedgraph file with CpG and corresponding methylation value. However, I am wondering …
These days read trimming is generally unnecessary in both DNA and RNAseq. Is there any reason why the same shouldn't be true of bisulfite-treated WGBS …
Hi, I have paired end R1.fastq, R2.fastq and singletons.fastq files for the same samples. What parameters should I use for aligning reads against a genome …
Hi, I have .fastq files from the same sample sequenced in two sequencing runs. First run was on Illumina Hiseq and generated paired-end 2x100bp reads. …
Changed your title to make it more specific, and added
biscuittag. Please think about this in the future, as such experts can more easily find your question.