I did this and I got :
:# All requested packages already installed.
But still I get an error that igv cannot load my sam file..
I want to use IGV on a server so I don't have to download bam files to my local machine. I used conda to install igvtools. When I type igvtools in the command line I get this error:
Using system JDK. Unrecognized option: --module-path=/home/kmmahan/miniconda3/envs/igv/share/igvtools-2.5.3-0/lib Error: Could not create the Java Virtual Machine. Error: A fatal exception has occurred. Program will exit.
Any help would be appreciated
If any one looking for the fix, install the latest openjdk using conda by conda install -c conda-forge openjdk and it works.
I did this and I got :
:# All requested packages already installed.
But still I get an error that igv cannot load my sam file..
Is your sam file sorted and indexed? Do you get this error when browsing in IGV GUI to select the file you are trying to view?
Yes , my sam file was sorted and indexed ,I fixed the problem by uploading the correct reference genome. I didnt know that the default refseq is the human genome. thats was my problem.
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IGV can be used on a server. Do you have
javainstalled on this server? IGV needs Java. Local installs requireJava 11.(igv) [kmmahan@blogin4 analysis]$ java -version openjdk version "1.7.0_91" OpenJDK Runtime Environment (Zulu 7.12.0.3-linux64) (build 1.7.0_91-b15) OpenJDK 64-Bit Server VM (Zulu 7.12.0.3-linux64) (build 24.91-b15, mixed mode)
So it is using JDK instead of JVM?
I am guessing that 1.7 is based on Java 7? If thats the case you will need to update this.
I am pretty sure that
igvtoolsis not the same thing as IGV.Command line version of IGV includes igvtools. That package requires
Java 11.