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FastQC SequenceFormatException Midline didn't start with '+'

uk.ac.babraham.FastQC.Sequence.SequenceFormatException: Midline '@A00818:33:HKYV3DSXX:4:2433:19217:4366 1:N:0:AGCCAATT' didn't start with '+' at uk.ac.babraham.FastQC.Sequence.FastQFile.

I used the solution for the previous posts.I get this: gzip: invalid compressed data--format violated Is my data originally damaged?

rna-seq software error fastqc
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updated by WouterDeCoster WouterDeCoster • written by 2899135311 • 2899135311

Please consider using a concise title. Posting an error message in title is not good etiquette.

Grep that header and see what the fastq record looks like: grep -A 3 "@A00818:33:HKYV3DSXX:4:2433:19217:4366" your_file. Third line in the fastq record should have +.

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written by GenoMax GenoMax

I just tried it, but there is no output. then i used the zgrep I got this error:

gzip: YS1_S1_L002_R1_001.fastq.gz: invalid compressed data--format violated

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written by 2899135311 • 2899135311

It is likely that your file is corrupted. Can you obtain a new copy?

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written by GenoMax GenoMax

Ok, I will go to get a copy of the data, thank you.

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written by 2899135311 • 2899135311

1 answer

I just tried it, but there is no output.

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written by 2899135311 • 2899135311

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