what do digitisation, offset, and range values mean in fast5 file of Nanopore sequencing

Question

[kumajis]

I recently downloaded an ONT Nanopore sequencing, but I do not know what do this values mean

 digitisation, offset, and range values

could anyone give me some brief answer?

Thanks

Answer 1

[Lhl]

The current intestiy values are transformed to intergers to save space of storage. To transform them back to raw, you will need these digitisation, offset, and range values.

Have a look at https://github.com/nanoporetech/fast5_research/blob/52a0b92e3bae0a6bbf3b61b2bde670c65827bfb0/fast5_research/fast5.py#L437